Feel free to add new protocols to the appropriate section or add comments to any existing protocol. Here we provide an overview of the current state of knowledge and resources for the study of this fish, with comments on the future direction of zebrafish genomics from professor mark fishman and dr stephen wilson. Research emphasis and objectives the zebrafish international resource center zirc is housed on the university of oregon campus in eugene. Chapter 19 zebrafish informatics and the zfin database monte westerfield institute of neuroscience university of oregon eugene, oregon 97403 eckehard doerry, arthur e. Regular care and maintenance of a zebrafish danio rerio. A guide for the laboratory use of zebrafish danio rerio rep spiral by westerfield, monte isbn. A guide for the laboratory use of zebrafish danio rerio, 3rd ed, univ. Simple method for steady, lowlevel embryo production. Its use has increased exponentially with the characterization of novel genes involved in vertebrate development and disease. The need to develop standardized diets to support zebrafish danio rerio research is supported by the knowledge that specific dietary ingredients, nutrients, or antinutritional factors in diets have been shown to affect development and growth of adult. However, the size of the zebrafish eggs dictated the sizing of the injecting and holding pipettes and affected ease of manipulation.
Zebrafish informatics and the zfin database 343 fig. Remove embryos from their chorions by immersing them in 1 mgml pronase for 5 minutes, room temperature, in a petri dish. Essential zebrafish methods by monte westerfield overdrive. Zebrafish are now gaining popularity in genetics, pharmacological and behavioural research. Pdf breeding of zebrafish in the laboratory environment. Cellular and developmental biology, part b developmental biology. A guide for the laboratory use of zebrafish brachydanio rerio monte westerfield. Douglas computer and information science department university of oregon eugene, oregon 97403 i. Due to its prolific reproduction and the external development of the transparent embryo, the zebrafish is the prime model for genetic and developmental studies, as well as research in genomics. Chapter 19 zebrafish informatics and the zfin database. This book, one of two new volumes in the reliable lab solutions series dealing with zebrafish, brings together a robust and uptodate collection of timetested methods presented by the worlds leading scientists. Toxicity evaluation of acrylamide on the early life stages of the zebrafish embryos danio rerio. The zebrafish book a guide for the laboratory use of zebrafish brachydanio rerio by monte westerfield.
A guide for the laboratory use of zebrafish danio rerio monte westerfield on. As a vertebrate, zebrafish share considerable genetic sequence similarity. Wildcard searching if you want to search for multiple variations of a word, you can substitute a special symbol called a wildcard for one or more letters. Basically the same equipment used for icsi in mammals was used in zebrafish. Open library is an open, editable library catalog, building towards a web page for every book ever published. This material is from the 4th edition of the zebrafish book.
This book was originally published by monte westerfield in 1993 and printed by the university of oregon press. Zebrafish international resource center zirc protocols. Protein purification and western blot detection from. The zebrafish book a guide for the laboratory use of zebrafish danio rerio rep spiral edition by monte westerfield. George streisinger in the zebrafish book, a guide for using zebrafish in the laboratory westerfield 2000. The zebrafish book a guide for the laboratory use of zebrafish danio brachydanio rerio by monte westerfield, institute of neuroscience, university of oregon. Hardcopies of the 4th edition of the zebrafish book can be obtained for a nominal fee from the zebrafish international resource center, 5274 university of oregon, eugene, or 97403 usa. Due to the covid19 pandemic, resource distribution has been suspended and services may be delayed. The zygote within its uplifted chorion, a few minutes after fertilization. Beginning in the early days of zebrafish research, several resources were developed, including the zebrafish book, dedicated to george streisinger. Chapters cover such topics as genetrap mutagenesis, genetic screens for mutations, gene editing in zebrafish, homologous gene targeting, genomewide rna tomography, and. Zebrafish basics zebrafish, danio rerio, is a tropical fish belonging to the order cypriniformes family cyprinidae genus daniozebrafish belong to the same family as carp and minnow and are indigenous to areas within the himalayan region, including pakistan, myanmar, nepal, and india 1,2.
A guide for the laboratory use of zebrafish danio rerio, published in 2007, may be ordered from the zebrafish international resource center, zirc. Streisinger, in westerfield, 1994 and incubated at an optimal temperature with out crowding 28. Breeding of zebrafish in the laboratory environment for research development article pdf available in bangladesh journal of pharmacology 124. We will contact you to arrange shipments when normal operations are resumed. Streisinger devised approaches to identify mutations in zebrafish without extensive breeding that became the framework for the first genetic screens. Everyday low prices and free delivery on eligible orders. Phrase searching you can use double quotes to search for a series of words in a particular order. Zebrafish maintenance zebrafish were maintained essentially as described in westerfield. For example, world war ii with quotes will give more precise results than world war ii without quotes.
Aug 25, 2014 westerfield m 2000 the zebrafish book. The journal will serve as a forum for papers discussing research on comparative genomics and evolution, the genetic. For continuous production of a relatively small number of embryos 3050 per tank per day, use an equal number of males longer, slimmer, and more yellow especially on the belly and females plumper and more silvery. The laboratory zebrafish crc press book like other books in the laboratory animal pocket reference series, this guide covers all aspects pertaining to the use of these organisms including their basic biology, humane care and management, husbandry, life support systems, regulatory compliance, technical procedures, veterinary care, and wate. The zebrafish book january 1994 edition open library. The zebrafish genetics, genomics, and transcriptomics h. A guide for the laboratory use of zebrafish danio rerio monte westerfield. While genetically distant from humans, nonetheless th.
Zebrafish are photoperiodic in their breeding, and produce embryos every morning, shortly after sunrise. Native to south asia, it is a popular aquarium fish, frequently sold under the trade name zebra danio and thus often called a tropical fish although both tropical and subtropical. Zon production of haploid and diploid androgenetic zebrafish including methodology for delayed in vitro. Search for library items search for lists search for contacts search for a library. Growth and survival of zebrafish danio rerio fed different commercial and laboratory diets. A guide for the laboratory use of zebrafish danio rerio westerfield, monte on. Download for offline reading, highlight, bookmark or take notes while you read essential zebrafish methods. A guide for the laboratory use of zebrafish danio rerio 4th.
Acknowledging materials and services please acknowledge the zebrafish international resource center in all publications resulting from the materials andor services we are providing to you. A guide for the laboratory use of zebrafish danio rerio 9789994860579 by westerfield, monte and a great selection of similar new, used and collectible books available now at great prices. Download it once and read it on your kindle device, pc, phones or tablets. Westerfield edition, in english the zebrafish book 1993 edition open library. Monte westerfield author of essential zebrafish methods. A guide for the laboratory use of zebrafish danio rerio, eugene, university of oregon press. Growth and survival of zebrafish danio rerio fed different.
The zebrafish danio rerio has recently been shown to be an ideal model to study bone disease including osteoporosis. All orders will be processed in the order received. Neurobehavioral studies in zebrafish are typically based on automated videotracking of individual or group fish responses to various stressors, drug treatments and genetic manipulations. Guide for the laboratory use of zebrafish danio rerio. Zon cell cycles and development in the embryonic zebrafish donald a. Asynchrony arises at the earliest stages, and it becomes more pro nounced as time passes. Appendix b the zebrafish handbook i westerfield 1993. Myosin heavy chain expression in zebrafish and slow muscle. Genetics, genomics, and transcriptomics, fourth edition, is the latest volume in the methods in cell biology series that looks at methods for the analysis of genetics, genomics, and transcriptomics of zebrafish. The zebrafish danio rerio is a freshwater fish belonging to the minnow family of the order cypriniformes. Cellular and developmental biology, part b issn book 4 kindle edition by detrich iii, h.
This protocol describes regular care and maintenance of a zebrafish laboratory. Quantitative microscopy of angiogenesis in zebrafish embryos. Culled from previously published chapters in methods in cell biology and updated by the original authors where relevant. Phosphorylation of the zebrafish m6ab at serine 263 contributes to filopodium formation in pc12 cells and neurite outgrowth in zebrafish embryos. Older literature referred to zebrafish as brachydanio rerio. The zebrafish book a guide for the laboratory use of zebrafish danio brachydanio rerio by monte westerfield, institute of neuroscience, university of oregon older literature referred to zebrafish as brachydanio rerio. Monte westerfield is the author of zebrafish book 4. A guide for the laboratory use of zebrafish danio rerio. Zebrafish orthologs of human genes that are implicated in human disease etiology are routinely. Extraction of proteins from zebrafish embryos for sdsgel. William detrich, iii, monte westerfield, leonard zon. Cell and developmental biology ebook written by monte westerfield, leonard i. The zebrafish book a guide for the laboratory use of. Wildtype zebrafish obtained from a local pet store were spawned as described by westerfield 17 and reared 28 days postfertilization on rotifers.
Pdf zebrafish in manual of animal technology researchgate. The data model specifies the attributes of each type of data. Product description type unit quantity academic price book. Protocols are organized into sections corresponding to the chapters of the zebrafish book, 5th edition 4th edition online. Disease models and chemical screens, volume 8 4th edition.
The 5th edition is available in print and within the zfin protocol wiki. Mar 12, 2020 this is where zebrafish researchers can share experimental protocols and tips with the rest of the research community. In order to collect embryos, male and female zebrafish were put into a breeder basket the night before and embryos were collected in the morning. Embryos younger than 24 hpf require shorter exposure to pronase see removing embryos from their chorions. The zebrafish has long been a favourite model for the study of vertebrate development. Abstract background the zebrafish danio rerio is rapidly emerging as an important model species in neuroscience research. Every functional study eventually involves the demonstration of differential expression or activity of a protein of interest. A paperback version of the fifth edition, called the zebrafish book. A guide for the laboratory use of zebrafish danio brachydanio rerio. The zebrafish osteoporosis model could be induced by glucocorticoid treatment with chemical staining for reflecting the level of bone mineralization.
824 1532 993 415 576 122 504 460 96 604 419 155 704 240 616 1473 282 222 1578 23 225 1010 333 184 1275 1376 1276 1174 944 1063 1530 718 880 770 735 1060 203 587 1484 383 1299 447 322 1140 889